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Research Outline      2015-12-14 10:50:53

I have made MDCK (Madin-Darby canine kidney) cells expressing small GTPase RhoA, Rac1, and Cdc42 mutants under a tightly regulated expression system which allows a very useful model to study the effects of RhoA, Rac1, and Cdc42 on cell growth, differentiation, cell-substratum adhesion, cell-cell adhesion, cell motility, and cell polarity formation. In deed, I found the expression of RhoA and Rac1 mutant could affect the polarity formation of epithelial cells and also the formation of tight junction in MDCK cells. To continue these works, I am planning to explore the following areas:

(1) Anoikis (Apoptosis of epithelial cells induced by the loss of contact with substratum) and the contribution of RhoA, Rac1, and Cdc42 to the signaling pathway involved in anoikis.

(2) Hypoxia induced signaling pathway. Since Rac1 is a component of the multi-protein complex, NADPH, and involved in oxygen burst in microphage. The role of Rac1 in the context of oxidative stress and reperfusion tissue injury would be explored.

(3) Cloning of GP135, an apical membranous protein, which is specifically expressed in the thick ascending limb of loop of Henle. Because the possibility of this protein as a channel, or transporter, or ionic pump, I would like to clone the cDNA sequence to study the function of this protein.